Results.

 

THE EFFECTIVENESS OF PTAT TECHNOLOGY IS PROVEN IN SEVERAL FIELDS OF USE

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RESULTS:

PTAT TECHNOLOGY IMPROVES CRYOTOLERANCE OF HUMAN SEMEN

The cryotolerance of normozoospermic semen samples (n=11) from patients visiting IVF centres was investigated. The post-thaw motility was higher after PTAT preconditioning (p<0.05).

 

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INCREASED SURVIVAL RATE OF STORAGE-COOLED BOAR SEMEN AFTER PTAT TREATMENT

The total motility of boar semen stored at 15°C has increased due to the PTAT preconditioning (n=7).

After inseminations healthy piglets were born and the PTAT preconditioning resulted in an increased litter size.

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INCREASED CRYOTOLERANCE OF BOAR SEMEN DUE TO PTAT PRECONDITIONING

PTAT technology has a significant beneficial effect on boar semen cryosurvival: after thawing the total and progressive motility increased compared to the control samples frozen by the standard procedure (34 ejaculates of 4 boars, p<0.001).

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PTAT PRECONDITIONING INCREASES THE REPRODUCTIVE PERFORMANCE OF FROZEN BOAR SEMEN

Fertility rate and litter size increased after inseminations with PTAT treated frozen boar semen (34 ejaculates of 4 boars, 102 inseminations).

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INCREASED CRYOSURVIVAL OF BULL SEMEN AFTER PTAT PRECONDITIONING

The PTAT treatment has a beneficial effect on bull semen, the post-thaw motility is significantly higher (34 ejaculates).

 

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PTAT INCREASES POST-THAW SPERMATOZOA SURVIVAL OF STALLION

PTAT preconditioning improves the recovery rate of the stallion semen freezing by preserving viability and function of the spermatozoa. Accordingly, it can increase the effectiveness of stallion semen cryopreservation and create value in the horse breeding industry.

 

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INCREASED SURVIVAL RATE OF VITRIFIED HUMAN OOCYTES AFTER PTAT PRECONDITIONING

PTAT pre-treatment can enhance the cryosurvival of vitrified human oocytes (n=306).

 

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PTAT TREATMENT ENHANCES THE DEVELOPMENTAL COMPETENCE OF VITRIFIED HUMAN OOCYTES

The post-thaw oocyte activation rate increased due to the PTAT preconditioning (n=306).

 

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PTAT TREATMENT INCREASED THE POST-THAW DEVELOPMENTAL COMPETENCE OF PORCINE OOCYTES

The cleavage rate and the blastocyst rate of PTAT preconditioned vitrified porcine oocytes (n=201) were significantly higher compared to control vitrified oocytes (n=142, p<0.05). The control oocytes cleaved but could not reach the blastocyst stage.

 

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PTAT PRECONDITIONING IMPROVES THE QUALITY OF PORCINE OOCYTES AFTER CLONING

After somatic cell nuclear transfer (SCNT) and in vitro culturing the blastocyst rate significantly increased, when PTAT treated (n=144) porcine oocytes were compared to control oocytes (n=204). Additionally, after vitrification the survival rate of the cloned blastocysts was higher (p<0.01).

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PTAT TREATMENT OF PORCINE OOCYTES IMPROVED THE EFFICACY OF IN VITRO MATURATION

PTAT preconditioning of GV stage porcine oocytes (n=825) enhanced the blastocyst rate and blastocyst cell number.

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INCREASED IN VITRO DEVELOPMENTAL COMPETENCE OF VITRIFIED MOUSE OOCYTES AFTER PTAT TREATING

PTAT preconditioning before vitrification improved the in vitro and in vivo developmental competence of mouse oocytes:

- after ICSI (intra-cytoplasmic sperm injection) increased cleavage rate
– after embryo transfer higher birth rate was detectable, compared to control vitrified oocytes (n=358)

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PTAT PRECONDITIONING INCREASES THE SURVIVAL RATE OF VITRIFIED BOVINE EMBRYOS

PTAT preconditioning before vitrification improved the re-expansion rate of bovine embryos (n=120).

Due to the PTAT preconditioning the number of „freezable embryos” increased.

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PTAT PRECONDITIONING OF VITRIFIED BOVINE EMBRYOS RESULTS IN IMPROVED DEVELOPMENTAL COMPETENCE

PTAT pre-treated vitrified bovine embryos showed higher hatching rate compred to the control vitirified embryos (n=404).

Due to the PTAT preconditioning the number of „freezable embryos” increased.

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INCREASED POST-THAW EMBRYO QUALITY DUE TO THE PTAT PRECONDITIONING OF VITRIFIED OVINE EMBRYOS

PTAT preconditioning before vitrification improves the hatching rate and decreases the picnotic index of ovine embryos
(p<0.05).

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AFTER THE PTAT TREATMENT THE QUALITY OF IN VITRO CULTURED OVINE EMBRYOS IS BETTER

PTAT preconditioning of ovine embryos improves the efficacy of in vitro culturing: higher total cell number (p<0.01) and lower number of picnotic nuclei (p<0.05) was detectable.

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AFTER PTAT PRECONDITIONING THE SURVIVAL RATE OF CHILLED ZEBRAFISH EMBRYOS IS HIGHER

After 24 hours chilling zebrafish embryos showed increased survival rate when PTAT pretreated (n=786) and control embryos (n=806) were compared.

At 30 and 60 days post-fertilization no more survivor in the control group while some PTAT treated fish still alive.

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PTAT RESULTS IN INCREASED CRYOSURVIVAL OF HUMAN CORD BLOOD STEM CELLS

After PTAT treatment the cryopreserved human cord blood cells showed improved survival rate (n=9): in the PTAT treated group the ratio of viable white blood cells and CD34+/CD45+ stem cells were significantly higher compared to the control (p<0.05)
The DNA fragmentation was lower in the PTAT treated samples*

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PTAT TREATMENT OF MOUSE EMBRYONIC STEM CELLS IMPROVED THE CRYOSURVIVAL

Vitrification of PTAT preconditioned mouse embryoid bodies resulted in increased survival rate compared to control (n=176).

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THE DIFFERENTIATION POTENTIAL OF VITRIFIED MOUSE EMBRYONIC STEM CELLS IMPROVED AFTER PTAT TREATMENT

Vitrified mouse embryonic bodies showed increased differentiation potential toward cardiac lineages when PTAT preconditioning was applied (n=156; p<0.05).

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PTAT TREATMENT OF MICROBES RESULTS IN IMPROVED SURVIVAL RATE OF LYOPHILIZATION

PTAT preconditioning of lyophilized Lactobacillus and Escherichia Coli species increased the survival rate and viable cell number.

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